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[Bacillus subtilis mutants resistant to roseoflavin].

A Ia Kukanova, V G Zhdanov, A I Stepanov

    Genetika
    |February 1, 1982
    PubMed
    Summary

    Bacillus subtilis mutants resistant to roseoflavin excrete riboflavin. Combining specific mutations (rosR and ribC) in Bacillus subtilis hyper-regulates riboflavin production.

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    [Transketolase mutation in riboflavin-synthesizing strains of Bacillus subtilis].

    Molekuliarnaia genetika, mikrobiologiia i virusologiia·2000

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Context:

    • Bacillus subtilis is a model organism for studying microbial metabolism and genetic regulation.
    • Riboflavin (vitamin B2) biosynthesis is a complex pathway regulated by specific genes.
    • Roseoflavin is a structural analog of riboflavin used to select for resistant mutants.

    Purpose:

    • To investigate the genetic basis of roseoflavin resistance in Bacillus subtilis.
    • To characterize the mutations responsible for altered riboflavin excretion.
    • To explore strategies for enhancing riboflavin overproduction.

    Summary:

    • Mutants of Bacillus subtilis exhibiting resistance to high concentrations of roseoflavin were identified.
    • These resistant mutants excrete significant amounts of riboflavin, along with minor quantities of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD).
    • Mutations conferring roseoflavin resistance (rosR) were mapped to the operator region of the riboflavin operon, suggesting a direct role in regulating gene expression.
    • The combined effect of rosR mutations and mutations in the ribC gene (a regulator gene) resulted in hyperproduction of riboflavin.

    Impact:

    • Elucidates the regulatory mechanisms governing the riboflavin operon in Bacillus subtilis.
    • Identifies specific genetic targets for metabolic engineering to enhance riboflavin yields.
    • Provides a foundation for developing improved microbial strains for industrial riboflavin production.

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