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Modified Dupont aca calcium method for hemolyzed specimens.

R G Haas, S Mushel

American Journal of Clinical Pathology

|February 1, 1982

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Hemoglobin in samples causes falsely high calcium readings on the DuPont aca analyzer. A modification using EGTA allows for accurate calcium measurement in hemolyzed samples by correcting for hemoglobin interference.

Area of Science:

Clinical Chemistry
Analytical Chemistry
Biochemistry

Background:

Hemoglobin presence in serum or plasma interferes with calcium determination.
The DuPont Automatic Clinical Analyzer (aca) exhibits increased apparent calcium concentration with hemoglobin presence.

Purpose of the Study:

To address the positive interference of hemoglobin on calcium measurements.
To develop a method for accurate calcium quantification in hemolyzed samples.

Main Methods:

A pack modification utilizing ethylene glycol-bis-(2-aminoethylether)-tetracetic acid (EGTA) was implemented.
EGTA facilitates the estimation of positive interference caused by hemoglobin color.

Main Results:

The EGTA modification allows for the estimation of hemoglobin-induced positive interference.

Related Experiment Videos

A correction factor was developed to adjust for this interference.

Conclusions:

Accurate calcium measurement in hemolyzed samples is achievable with the described method.
The EGTA pack modification provides a reliable solution for calcium analysis in the presence of hemoglobin.