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Related Experiment Videos

DNA replication is required for tissue-specific enzyme development in ascidian embryos.

N Satoh

    Differentiation; Research in Biological Diversity
    |January 1, 1982
    PubMed
    Summary

    Ascidian embryos require multiple DNA replications for histospecific enzyme development. Inhibiting DNA synthesis early prevents tyrosinase and alkaline phosphatase formation, but later inhibition allows enzyme differentiation.

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    Area of Science:

    • Developmental Biology
    • Molecular Biology
    • Genetics

    Background:

    • Ascidian embryos exhibit tissue-specific enzyme synthesis during development.
    • Tyrosinase and alkaline phosphatase are key enzymes studied for their developmental roles.
    • Gene activation and maternal mRNA are implicated in enzyme production.

    Purpose of the Study:

    • To investigate the role of DNA replication in the development of histospecific enzymes in ascidian embryos.
    • To determine the critical stages of DNA synthesis required for enzyme differentiation.

    Main Methods:

    • Ascidian embryos were treated with aphidicolin, a DNA synthesis inhibitor, at various cleavage stages.
    • Embryos were arrested at different developmental stages to assess enzyme production.

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  • Histochemical detection was used to identify the activity of tyrosinase and alkaline phosphatase.

    • Main Results:

    • Early cleavage stage arrest (up to 64-cell for tyrosinase, 16-cell for alkaline phosphatase) with aphidicolin prevented enzyme differentiation.
    • Embryos arrested at later stages (early gastrulae for tyrosinase, 32-cell for alkaline phosphatase) were still able to differentiate enzymes.
    • These findings indicate a requirement for multiple DNA replications prior to enzyme development.

    Conclusions:

    • DNA replication is essential for the timely development of histospecific enzymes in ascidian embryos.
    • The timing of DNA replication relative to developmental stage is crucial for enzyme differentiation.
    • These results highlight the dependence of enzyme development on cell division cycles.