Electron microscopic demonstration of negative surface charges on cytoplasmic membranes of Bacillus subtilis with...

The application of a protamine-ferritin conjugate for labelling of isolated protoplast membranes of Bacillus subtilis S 13/1 is described. Contrary to Mycoplasma membranes which could only be labelled on the outer side of the membrane, ferritin was deposited on both membrane sides as a single layer without cluster formation.