Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The phospholipase A2 from human platelets.

R Apitz-Castro, M R Cruz, M Mas

    Agents and Actions
    |December 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Alexithymia Moderates Salience Effects in Emotional Facial Expression Perception and Recognition.

    International journal of psychology : Journal international de psychologie·2025
    Same author

    A DFT approach towards understanding the thermal stability of TKX-50 and their key precursors through band gaps and MESP.

    Journal of molecular modeling·2022
    Same author

    Comparison of INSAT-3D retrieved total column ozone with ground-based and AIRS observations over India.

    The Science of the total environment·2021
    Same author

    Correlation between thermoluminescence and optically stimulated luminescence responses of natural alexandrite.

    Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2020
    Same author

    Complete remission with immunotherapy: Case report of a patient with metastatic bladder cancer to the humerus.

    Urology case reports·2020
    Same author

    Understanding the relationship between soil properties and litter chemistry in three forest communities in tropical forest ecosystem.

    Environmental monitoring and assessment·2020
    Same journal

    Classification and biological distribution of histamine receptor sub-types. 1975.

    Agents and actions·1994
    Same journal

    Metiamide--an orally active histamine H2-receptor antagonist. 1973.

    Agents and actions·1994
    Same journal

    A brief survey of the history of inflammation. 1978.

    Agents and actions·1994
    Same journal

    The discovery of librium. 1972.

    Agents and actions·1994
    Same journal

    Notes and documents concerning the discovery of LSD. 1970.

    Agents and actions·1994
    Same journal

    Inhibition of 5-hydroxytryptamine-induced and -amplified human platelet aggregation by ketanserin (R 41,468), a selective 5-HT2-receptor antagonist. 1982.

    Agents and actions·1994
    See all related articles

    Researchers purified human platelet phospholipase A2 (PLA2), finding it prefers unsaturated phospholipids and is activated by products, suggesting a role in arachidonic acid release.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Platelet Biology

    Background:

    • Phospholipase A2 (PLA2) plays a crucial role in cellular signaling.
    • Understanding human platelet PLA2 is vital for elucidating platelet function and related pathologies.

    Purpose of the Study:

    • To characterize a purified human platelet phospholipase A2 (PLA2).
    • To investigate the substrate specificity and activation mechanisms of human platelet PLA2.

    Main Methods:

    • Purification of human platelet phospholipase A2 (PLA2).
    • Enzyme activity assays under varying conditions (pH, substrate form, product presence).
    • Determination of molecular weight and cofactor requirements.

    Main Results:

    Related Experiment Videos

  • Purified human platelet PLA2 has a MW of ~50 K Dalton, requires Ca++, and has a pH optimum of 9.4.
  • Unsaturated phospholipids are preferred substrates, with higher activity on aggregated forms.
  • Enzyme activity is significantly influenced by substrate interface quality and stimulated by reaction products.

    • Conclusions:

    • Human platelet PLA2 exhibits unique properties regarding substrate preference and activation.
    • Product-mediated activation suggests a role in stimulus-linked arachidonic acid release.
    • Potential involvement of PLA2 activation by phospholipase C pathway intermediates in platelet function.