Immobilization of the periplasmic maltose-binding protein of Escherichia coli

Easy and rapid procedures for immobilization of the periplasmic maltose-binding protein was worked out. The matrix was able to adsorb a maltose-chemosensing component, methyl-accepting chemotaxis protein of tar pathway. The adsorption was not seriously affected by the presence of other chemicals in tar pathway such as aspartate or nickel ion.