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A purified human diploid cell rabies vaccine
Developments in Biological Standardization
|January 1, 1978
Summary
Continuous-flow isopycnic banding purified rabies virus antigen for a human diploid cell rabies vaccine. The vaccine demonstrated high potency, averaging 234 antigenic values per milligram of protein before stabilization.
Area of Science:
- Virology
- Vaccine Development
- Biotechnology
Background:
- Rabies virus poses a significant global health threat.
- Effective vaccine production is crucial for rabies prevention.
- Purification of viral antigens is a key step in vaccine manufacturing.
Purpose of the Study:
- To describe a method for purifying rabies virus antigen.
- To evaluate the potency of a human diploid cell rabies vaccine.
- To present data on vaccine potency and stability.
Main Methods:
- Continuous-flow isopycnic banding in sucrose gradient for rabies virus antigen purification.
- Preparation of a human diploid cell rabies vaccine using purified antigen.
- Measurement of vaccine specific potency (antigenic values per mg of protein).
Main Results:
- The purification method yielded rabies virus antigen suitable for vaccine preparation.
- The average specific potency of vaccine batches was 234 antigenic values per 1.0 mg of protein before stabilizer addition.
- Additional data on vaccine potency and stability were collected and are presented.
Conclusions:
- Continuous-flow isopycnic banding is an effective method for purifying rabies virus antigen.
- The resulting human diploid cell rabies vaccine exhibits high initial potency.
- The study provides essential data for the development and quality control of rabies vaccines.