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A human plasma cell line: induction and characterization.

C H Miller, A Carbonell, R Peng

    Cancer
    |May 15, 1982
    PubMed
    Summary
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    A new stable human plasma cell line was created from bone marrow, producing IgG kappa. This cell line aids in studying human myeloma immunobiology.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Limited availability of human plasma cell lines for research.
    • Need for reliable models to study multiple myeloma.

    Purpose of the Study:

    • To establish and characterize a stable human plasma cell line.
    • To assess its utility in immunobiological studies of human myeloma.

    Main Methods:

    • Established cell line from myelomatous human bone marrow using conditioned media.
    • Cultured cells in RPMI 1640 with fetal calf serum under 6% CO2.
    • Performed surface marker analysis, transmission electron microscopy, radioimmunoassay, and ASG banding.

    Main Results:

    • Achieved a 62-hour doubling time at optimal cell concentration.

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  • Cells expressed Fc receptors (83%) and variable complement receptors, lacking sheep erythrocyte receptors and surface immunoglobulins.
  • Demonstrated IgG kappa production (23 ng IgG, 28.7 ng Kappa per 1x10^6 cells in 72 hours).
  • Cells were negative for lipase, esterase, and Epstein-Barr nuclear antigen; exhibited variable chromosome numbers (46-49).

    • Conclusions:

    • A stable, IgG kappa-producing human plasma cell line (RPMI 4098) has been successfully established.
    • The characterized cell line provides a valuable tool for advancing immunobiological research in human myeloma.