Related Experiment Video
Updated: May 11, 2026

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Published on: June 12, 2013
Cell wall-defective variants of pseudomonas-like (group Va) bacteria in Crohn's disease
Abstract:
Several animal transmission studies have indicated that Crohn's disease may be caused by a filterable agent. Filtrates of homogenized tissues were prepared from 8 patients with Crohn's disease, 9 patients with chronic ulcerative colitis, and 20 control patients without inflammatory bowel disease. Conventional bacteriological cultures and hypertonic cultures for cell wall-defective bacterial variants were performed on the filtrates. Bacterial revertants (parent forms) of cell wall-defective variants were obtained from filtrates of various tissues including mesenteric lymph nodes of all patients with Crohn's disease. In no instance were revertants cultured from tissue filtrates of the other patients. The 11 revertants isolated from the 8 patients were identified as Pseudomonas-like bacteria, most closely identifiable with group Va.
More Related Videos
08:24Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
08:34Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Published on: January 8, 2020
Related Concept Videos
Plant Cell Wall
Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by transmural...
Bacterial Cell Wall
Archaeal Cell Wall
Inflammatory Bowel Disease III: Crohn's Disease
Diverticular Disease of the Colon