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Related Experiment Videos

A solid phase fluoroimmunoassay of serum cortisol.

Y Kobayashi, M Yahata, F Watanabe

    Journal of Steroid Biochemistry
    |April 1, 1982
    PubMed
    Summary

    A new direct solid phase fluoroimmunoassay for serum cortisol offers a sensitive and accurate method for routine clinical testing. This assay provides reliable cortisol measurements comparable to radioimmunoassay, with good precision and accuracy.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Analytical Chemistry

    Background:

    • Accurate serum cortisol measurement is crucial for diagnosing adrenal disorders.
    • Existing methods like radioimmunoassay have limitations, necessitating development of alternative assays.

    Purpose of the Study:

    • To establish a direct solid phase fluoroimmunoassay for serum cortisol.
    • To evaluate the sensitivity, accuracy, precision, and applicability of the developed assay for routine use.

    Main Methods:

    • Utilized fluorescein isothiocyanate-labeled anti-cortisol antibody and cortisol-conjugated polyacrylamide beads.
    • Employed sodium salicylate as a blocking agent for cortisol-binding proteins.
    • Validated the assay's performance through sensitivity, measurable range, and recovery studies.

    Main Results:

    • Achieved a sensitivity of 0.2 ng/assay tube with a measurable range of 2-100 µg/dL.
    • Demonstrated good intra-assay (11.6%) and inter-assay (12.1%) coefficients of variation.
    • Showed high accuracy (100.2% average recovery) and strong correlation with radioimmunoassay (r=0.98).

    Conclusions:

    • The developed direct solid phase fluoroimmunoassay is a sensitive, accurate, and precise method for serum cortisol determination.
    • The assay meets standard criteria for dilution, accuracy, and precision.
    • This fluoroimmunoassay is suitable for routine serum cortisol measurements in clinical settings.

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