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Carbohydrate fermentation by Clostridium difficile.

S Nakamura, S Nakashio, K Yamakawa

    Microbiology and Immunology
    |January 1, 1982
    PubMed
    Summary
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    This study simplifies identifying Clostridium difficile by showing that standard media and a 2-day incubation are effective for biochemical tests, including mannitol fermentation and gelatin liquefaction.

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • Accurate identification of Clostridium difficile is crucial for clinical laboratories.
    • Standardized biochemical testing aids in reliable organism differentiation.

    Purpose of the Study:

    • To re-evaluate the biochemical properties of Clostridium difficile for practical laboratory identification.
    • To establish simplified and effective methods for routine diagnostics.

    Main Methods:

    • Culturing Clostridium difficile strains in a modified proteose peptone medium with L-cysteine.HCl and agar.
    • Assessing bacterial growth and fermentation of various sugars (fructose, glucose, mannitol, mannose, melezitose, sorbitol).
    • Evaluating gelatin liquefaction capabilities of the tested strains.

    Main Results:

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    • Modified medium with L-cysteine.HCl and agar supported sufficient growth for fermentation analysis.
    • A 2-day incubation period was adequate for determining sugar fermentation patterns.
    • All 82 Clostridium difficile strains tested liquefied 2% gelatin but not 10% gelatin.
    • Mannitol fermentation and gelatin liquefaction are key distinguishing features.

    Conclusions:

    • Simplified culture conditions and incubation times are effective for Clostridium difficile identification.
    • Mannitol fermentation combined with gelatin liquefaction reliably differentiates Clostridium difficile from other clostridia.
    • These findings facilitate practical and efficient laboratory diagnostics for Clostridium difficile.