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Related Experiment Videos

An improved acriflavine-Feulgen method.

J W Levinson, S Retzel, J J McCormick

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |May 1, 1977
    PubMed
    Summary
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    A modified acriflavine-Feulgen method simplifies deoxyribonucleic acid staining. This technique enhances nuclear contrast and may improve DNA quantification using cytofluorometry.

    Area of Science:

    • Histochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • The Feulgen reaction is a standard method for detecting deoxyribonucleic acid (DNA).
    • Traditional acriflavine-Feulgen staining can involve complex reagent preparation and may result in non-specific cytoplasmic staining.
    • Improved methods are needed for precise DNA quantification, particularly for techniques like cytofluorometry.

    Purpose of the Study:

    • To modify the acriflavine-Feulgen staining method for enhanced deoxyribonucleic acid (DNA) histochemistry.
    • To simplify reagent preparation and improve staining specificity.
    • To assess the utility of the modified method for quantitative DNA analysis.

    Main Methods:

    • Hydrolyzed cells were stained with 0.01% acriflavine in 90% ethanol.

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  • The modified staining solution eliminated poorly defined reagents.
  • Staining properties were evaluated in both hydrolyzed and unhydrolyzed cells.

    • Main Results:

    • The modified method simplified the preparation of the acriflavine-Feulgen reagent.
    • Hydrolyzed cells showed bright green staining of nuclei with minimal cytoplasmic staining.
    • Unhydrolyzed cells exhibited negligible staining of both cytoplasm and nuclei.
    • The technique demonstrated high specificity for stained nuclei.

    Conclusions:

    • The modified acriflavine-Feulgen method offers a simplified and specific approach for deoxyribonucleic acid (DNA) histochemistry.
    • The distinct nuclear staining and minimal background staining make it suitable for quantitative DNA analysis.
    • This improved technique holds potential for applications in cytofluorometry and other quantitative assays.