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Specific restoration of delayed hypersensitivity by lymphoid tissue extracts
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1977
Summary
Mice can regain their delayed hypersensitivity (DH) immune response after losing it. Cell-free spleen lysates from mice with active DH restored this immune function, suggesting a biological basis for transfer factor.
Area of Science:
- Immunology
- Cellular Immunology
- Hypersensitivity Reactions
Background:
- Mice exhibit a loss of demonstrable delayed hypersensitivity (DH) to various antigens.
- Restoring immune responsiveness is crucial for understanding immune memory and function.
Purpose of the Study:
- To investigate the potential for reconstituting lost delayed hypersensitivity (DH) in mice.
- To identify the components responsible for restoring immune responsiveness.
Main Methods:
- Mice were sensitized to antigens like DNFB, picryl chloride, and sheep red blood cells.
- Cell-free splenic and peritoneal exudate cell lysates from sensitized mice were administered.
- Assays were performed to measure the restoration of DH to related and unrelated antigens.
Main Results:
- Cell-free splenic lysates from mice with active DH restored immune responsiveness to structurally related antigens.
- Lysates were heat-labile (unstable at 56°C), not sedimented at 90,000 x G, and inactivated by trypsin or magnesium ions.
- Sera and lymphoid extracts from unsensitized mice showed no restorative activity.
Conclusions:
- A restorable form of unexpressed delayed hypersensitivity (DH) exists in mice.
- The properties of the restorative factor suggest a proteinaceous nature, potentially linked to the transfer factor phenomenon.