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Serial measurement of circulating immune complexes in healthy subjects
Insights
Circulating immune complex (CIC) levels fluctuate significantly in healthy humans and rats. Different measurement methods showed no correlation, indicating dynamic CIC composition even in health.
Area of Science:
- Immunology
- Clinical Chemistry
Background:
- Circulating immune complexes (CICs) are implicated in various physiological and pathological processes.
- Understanding baseline CIC levels and composition in healthy individuals is crucial for interpreting disease-related changes.
Purpose of the Study:
- To investigate the serial fluctuations of CIC levels in healthy human subjects and normal rats.
- To compare different methods for CIC detection and assess their correlation in healthy states.
Main Methods:
- Serial blood sampling from healthy humans and rats.
- Human CIC measurement using Clq solubility, complement consumption, and granulocyte phagocytosis assays.
- Rat CIC measurement using a modified complement consumption test.
Main Results:
- Significant fluctuations in CIC levels were observed in both healthy humans and rats over time.
- No consistent correlation was found between the results of the three different assays used for human CIC detection.
- This suggests that CIC levels are dynamic and their composition varies continuously in healthy individuals.
Conclusions:
- CIC levels are not static in healthy individuals and exhibit marked fluctuations.
- The composition of CICs appears to be dynamic, even in the absence of disease.
- Different assay methods may detect different aspects of CICs, highlighting the complexity of their measurement.
Abstract:
Circulating immune complex (CIC) levels were serially studied in healthy subjects and in normal rats. In human 3 methods (Clq solubility, complement consumption, and granulocyte phagocytosis tests) were used for the measurement of CIC; in rats, CIC levels were determined by the modified complement consumption test. A marked fluctuation in the CIC level was observed both in healthy human subjects and in normal rats. No correlation between results of the 3 assays used for CIC detection was found indicating that not only the level but the composition of CIC changes continuously in health.