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Related Experiment Videos

Reliability of disc diffusion susceptibility testing.

P R Murray, J R Zeitinger, D J Krogstad

    Infection Control : IC
    |May 1, 1982
    PubMed
    Summary
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    Retesting 2,181 bacteria-antibiotic combinations revealed interpretive changes in 5.5%, particularly for results near breakpoints. This highlights variability in antibiotic susceptibility testing and impacts clinical decisions.

    Area of Science:

    • Clinical Microbiology
    • Antimicrobial Resistance
    • Diagnostic Accuracy

    Background:

    • Accurate antibiotic susceptibility testing (AST) is crucial for guiding effective treatment.
    • Variability in AST results can lead to inappropriate antibiotic selection.
    • The Kirby-Bauer disc-diffusion method is a standard AST technique.

    Purpose of the Study:

    • To evaluate the frequency and nature of interpretive changes in bacteria-antibiotic combinations using the Kirby-Bauer method.
    • To identify factors associated with AST result variability, specifically focusing on zone diameter clustering near interpretive breakpoints.

    Main Methods:

    • Retesting of 2,181 bacteria-antibiotic combinations using the Kirby-Bauer disc-diffusion technique.
    • Analysis of zone diameter clustering around established interpretive breakpoints (within 2mm).

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  • Statistical assessment of the relationship between result clustering and reproducibility.

    • Main Results:

    • 120 (5.5%) of 2,181 combinations showed interpretive changes.
    • Most changes were single-step shifts (R to I, I to R/S, S to I).
    • Changes were significantly more frequent for combinations with zone diameters near breakpoints, showing a linear correlation between decreased reproducibility and increased clustering.

    Conclusions:

    • A notable proportion of bacteria-antibiotic combinations exhibit interpretive changes in AST.
    • Clustering of zone diameters near interpretive breakpoints is a key factor contributing to reduced reproducibility.
    • These findings underscore the importance of careful interpretation of AST results, especially for combinations near breakpoints.