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A major cytoplasmic glucose-regulated protein is associated with the Rous sarcoma virus pp60src protein
Abstract:
Previous studies have established that glucose deprivation of murine cells suppresses the synthesis of an Mr = 85,000 polypeptide. A protein of approximately the same molecular weight has been found to be associated with the transforming protein of Rous sarcoma virus, pp60src. The present study compares the Mr = 85,000 glucose-regulated protein with the pp60src-associated protein. By the criteria of mobility in sodium dodecyl sulfate-polyacrylamide gels and one-dimensional partial proteolytic peptide mapping the two proteins appear to be identical. It has previously been shown that the pp60src-associated protein is also identical with one of several proteins whose synthesis is induced after growth of cells at elevated temperatures or in the presence of arsenite and canavanine. Considering that it is involved in a number of complex response patterns, the name syndromin is proposed for this protein. These findings open the possibility of a fundamental interrelationship among the heat shock effect, regulation of protein synthesis by glucose deprivation, and oncogenic transformation by Rous sarcoma virus.
Insights
This study reveals that the 85,000 molecular weight glucose-regulated protein in murine cells is identical to the protein associated with Rous sarcoma virus
Area of Science:
- Molecular Biology
- Cell Biology
- Oncology
Background:
- Glucose deprivation in murine cells inhibits the synthesis of an 85,000 molecular weight (Mr) polypeptide.
- A similar Mr 85,000 protein is associated with the Rous sarcoma virus transforming protein, pp60src.
Purpose of the Study:
- To compare the Mr 85,000 glucose-regulated protein with the pp60src-associated protein.
- To investigate the potential interrelationship between cellular stress responses and viral oncogenesis.
Main Methods:
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to assess protein mobility.
- One-dimensional partial proteolytic peptide mapping to compare protein structures.
Main Results:
- The glucose-regulated protein and the pp60src-associated protein exhibit identical mobility in SDS-PAGE.
- Peptide mapping confirms the identity of the two proteins.
- The pp60src-associated protein is also identical to proteins induced by heat shock, arsenite, and canavanine.
Conclusions:
- The Mr 85,000 glucose-regulated protein is identical to the pp60src-associated protein, proposed to be named syndromin.
- These findings suggest a fundamental link between heat shock response, glucose regulation of protein synthesis, and Rous sarcoma virus-induced transformation.