Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lysine tRNAs from Bacillus subtilis 168: structural analysis.

B S Vold, D E Keith, M Buck

    Nucleic Acids Research
    |May 25, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

    Physical review letters·2026
    Same author

    Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Observation of tt[over ¯] Production in Pb+Pb Collisions at sqrt[s_{NN}]=5.02  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Search for Dark Matter Produced in Association with a Dark Higgs Boson in the bb[over ¯] Final State Using pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

    Physical review letters·2025
    Same author

    Search for Magnetic Monopole Pair Production in Ultraperipheral Pb+Pb Collisions at sqrt[s_{NN}]=5.36  TeV with the ATLAS Detector at the LHC.

    Physical review letters·2025

    Two Bacillus subtilis lysine tRNAs differ in anticodon loop modifications, impacting protein synthesis efficiency. These modifications are crucial for developmental changes in the bacteria.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Microbiology

    Background:

    • Lysine tRNA isoacceptors play vital roles in protein synthesis.
    • Post-transcriptional modifications in tRNA, particularly in the anticodon loop, can significantly affect tRNA function.
    • Bacillus subtilis exhibits developmental changes involving differential gene expression and protein synthesis.

    Purpose of the Study:

    • To establish the primary sequence of two lysine tRNA isoacceptors in Bacillus subtilis.
    • To investigate the differences in post-transcriptional modifications between these isoacceptors.
    • To correlate these modifications with their abundance and efficiency during bacterial development.

    Main Methods:

    • Primary sequence determination of lysine tRNA isoacceptors.

    Related Experiment Videos

  • Chromatographic separation to isolate different tRNA species.
  • Analysis of anticodon loop modifications using biochemical techniques.

    • Main Results:

    • Two lysine tRNA isoacceptors, tRNA lys 1 and tRNA Lys 3, were identified with identical primary sequences.
    • tRNA lys 1 exhibited an unmodified C at position 32 and a mixture of t6A and ms2t6A at position 37.
    • tRNA Lys 3, more efficient in protein synthesis, had a modified C at position 32 and only ms2t6A at position 37.

    Conclusions:

    • Differences in post-transcriptional modification, specifically at positions 32 and 37 of the anticodon loop, distinguish lysine tRNA isoacceptors in Bacillus subtilis.
    • These modifications influence tRNA efficiency in protein synthesis.
    • The modified tRNA Lys 3 may play a role in the functional interaction between translational and transcriptional regulation during Bacillus subtilis differentiation.