Effect of FSH deprivation on spermatogenesis in prepubertal mice

A specific antiserum to ovine follicle stimulating hormone (AS/FSH) raised in rabbits and administered to prepubertal mice from days 5 to 35 of age, inhibited intermediate and type B spermatogonia. Development of spermatocytes, spermatids, and spermatozoa was affected, indicating a role for FSH in the initiation of spermatogenesis. Decrease in the testicular and epididymal weights, but not that of seminal vesicles and ventral prostate, demonstrated the specific action of AS/FSH. Reduction in the weights of the epididymis in AS/FSH-injected mice accompanied by the absence of spermatozoa in the lumen suggests a possible role for FSH in spermiation.