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Migrant mu+ delta+ and static mu+ delta- B lymphocyte subsets
European Journal of Immunology
|July 1, 1982
Summary
Rats exhibit distinct B cell populations in spleen, lymph nodes, and blood. Most B cells in spleen and lymph nodes are mu+ delta-, while delta+ B cells migrate through the spleen.
Area of Science:
- Immunology
- Cell Biology
Background:
- B cells express various immunoglobulin isotypes, including mu and delta.
- B cell populations differ in distribution and function across lymphoid organs.
Purpose of the Study:
- To investigate the distribution and migratory properties of B cells expressing different immunoglobulin isotypes in rats.
- To differentiate between static and migrating B cell populations in lymphoid tissues and blood.
Main Methods:
- Flow cytometry and immunohistology were used to analyze B cell populations in rat spleen, lymph nodes, and blood.
- Selective depletion of migrating lymphocytes was achieved using splenic irradiation with a 32P-impregnated strip.
Main Results:
- Spleen marginal zones predominantly contain mu+ delta- B cells, while follicles contain mu+ delta+ B cells.
- Splenic irradiation selectively depleted delta+ B cells, indicating their migratory nature.
- A distinct mu+ delta- B cell population in the blood was resistant to splenic irradiation, suggesting an independent origin.
Conclusions:
- The major B cell population in rat spleen and lymph nodes is mu+ delta-.
- Delta+ B cells are highly migratory and traverse the spleen.
- A circulating mu+ delta- B cell population exists independently of splenic B cell migration.