Production and characterization of high titre rabbit antigonococcal R-type lipopolysaccharide serum

Two methods are reported for the production of rabbit anti-gonococcal lipopolysaccharide sera. One is produced by a conjugate between the core oligosaccharide and bovine serum albumin. The other is obtained by immunizing rabbits with heat-killed, ethanol-acetone washed bacteria. The specificity of the latter serum is studied, and it is shown that most of the antibodies are against a lactose-like determinant situated in the core region of the lipopolysaccharide. Preliminary studies show that this serum is able to cause specific agglutination of gonococci.