Enterococci. Biologic and epidemiologic characteristics and in vitro susceptibility

Area of Science:

• Microbiology
• Infectious Diseases
• Pharmacology

Background:

• Enterococci are significant pathogens causing urinary tract infections, endocarditis, and meningitis.
• Their role in polymicrobial infections is challenging to determine.
• Standard antibiotics show variable efficacy against Enterococci.

Purpose of the Study:

• To evaluate the antimicrobial susceptibility of Enterococci.
• To assess the synergistic activity of antibiotic combinations against Enterococci.

Main Methods:

• Antimicrobial susceptibility testing was performed using penicillin G, ampicillin, and vancomycin.
• The activity of various penicillins, cephalosporins, and piperacillin was assessed.
• Synergistic effects of combining cell wall active agents with aminoglycosides were investigated.

Main Results:

• Most Enterococci strains were inhibited by penicillin G (4 mg/L), ampicillin (2 mg/L), and vancomycin (6 mg/L).
• Piperacillin demonstrated activity comparable to penicillin G.
• Aminoglycoside addition to penicillin, ampicillin, vancomycin, or piperacillin resulted in synergistic activity, enhancing bactericidal effects.

Conclusions:

• Enterococci exhibit susceptibility to specific beta-lactams and vancomycin.
• Combination therapy with aminoglycosides is crucial for achieving synergistic and bactericidal activity against Enterococci.
• Understanding Enterococcal susceptibility patterns guides effective antimicrobial strategies.