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Related Experiment Videos

Confirmatory test for aflatoxin M1 on a thin layer plate.

H P Van Egmond, W E Paulsch, P L Schuller

    Journal - Association of Official Analytical Chemists
    |July 1, 1978
    PubMed
    Summary

    A simple trifluoroacetic acid (TFA) reaction confirms aflatoxin M1 identity on thin layer plates. This method accurately detects low levels of aflatoxin M1 and B1 in various food samples.

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    Mycotoxin research·2013

    Area of Science:

    • Food Safety
    • Analytical Chemistry
    • Mycotoxin Analysis

    Background:

    • Aflatoxin M1 is a significant foodborne contaminant.
    • Accurate detection and confirmation of aflatoxin M1 are crucial for public health.
    • Existing methods for aflatoxin M1 confirmation can be complex.

    Purpose of the Study:

    • To develop a simple and direct method for confirming aflatoxin M1 identity.
    • To validate the method's efficacy on real food samples.
    • To assess the method's sensitivity and applicability for simultaneous aflatoxin B1 confirmation.

    Main Methods:

    • Two-dimensional thin-layer chromatography (TLC) for aflatoxin separation.
    • On-plate reaction of aflatoxin M1 with trifluoroacetic acid (TFA).
    • Detection of blue fluorescence of the TFA derivative and comparison with M1 standards.

    Main Results:

    • The TFA reaction reliably confirmed aflatoxin M1 identity on the TLC plate.
    • The method successfully analyzed extracts from milk, cheese, and liver.
    • As little as 0.5 ng of aflatoxin M1 could be confirmed.
    • Simultaneous confirmation of aflatoxin B1 was also demonstrated.

    Conclusions:

    • Trifluoroacetic acid provides a straightforward and effective means for on-plate confirmation of aflatoxin M1.
    • This method enhances the reliability of aflatoxin M1 quantitation in food matrices.
    • The technique is sensitive, applicable to various food types, and can confirm both aflatoxin M1 and B1.

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