[Kinetics of the phosphorylase reaction under conditions of substrate inactivation]

Electrophoretically homogenous glycogenphosphorylase b was isolated from the Dasyatis pastinaca muscular tissue. The kinetic peculiarities of phosphorylase reaction in time were studied. It is found that the reaction rate substrate concentration real dependences are not described by the Michaelis equation. The fact of the irreversible inactivation of phosphorylase in the process of catalytic reaction is observed. It is supposed that this fact is mechanical destruction in the process of substrate splitting rather than thermoinactivation of the enzyme or its inhibition.