Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An improved method for determining total urinary phenol.

H E Spiegel, G E Opar

    Journal of Chromatography
    |August 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Continuous intravenous infusion pharmacokinetics of interferon to patients with leukemia.

    Journal of clinical pharmacology·1985
    Same author

    Intravenous and intramuscular pharmacokinetics of recombinant leukocyte A interferon.

    European journal of clinical pharmacology·1985
    Same author

    Pharmacokinetics of recombinant leukocyte A interferon following various routes and modes of administration to the dog.

    Journal of interferon research·1985
    Same author

    Improvement in clinical condition and thymus morphologic features associated with growth hormone treatment of immunodeficient dwarf dogs.

    American journal of veterinary research·1984
    Same author

    Interferon kinetics and adverse reactions after intravenous, intramuscular, and subcutaneous injection.

    Clinical pharmacology and therapeutics·1984
    Same author

    Pharmacokinetics of recombinant alpha A interferon following I.V. infusion and bolus, I.M., and P.O. administrations to African green monkeys.

    Journal of interferon research·1984
    Same journal

    Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash chromatography.

    Journal of chromatography·2013
    Same journal

    [Electrophoresis of radionuclides on cellogel].

    Journal of chromatography·2012
    Same journal

    Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.

    Journal of chromatography·2010
    Same journal

    Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993).

    Journal of chromatography·1994
    Same journal

    Determination of the calcium antagonist SIM6080 in plasma by high-resolution gas chromatography.

    Journal of chromatography·1994
    Same journal

    Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.

    Journal of chromatography·1994
    See all related articles

    A new, simple method accurately measures total urinary phenol using enzymatic hydrolysis and gas-liquid chromatography. This technique enhances the analysis of phenol levels in biological samples.

    Area of Science:

    • Analytical Chemistry
    • Biochemistry
    • Environmental Health

    Background:

    • Urinary phenol levels are important biomarkers for assessing exposure to various compounds.
    • Existing methods for total urinary phenol determination can be complex or time-consuming.

    Purpose of the Study:

    • To describe a simple, easily manipulated method for the accurate determination of total urinary phenol.
    • To provide a reliable analytical technique for routine laboratory use.

    Main Methods:

    • Enzymatic hydrolysis of conjugated phenols in urine.
    • Extraction of phenols into isopropyl ether.
    • Quantitation using gas-liquid chromatography with flame ionization detection (GC-FID).
    • Utilization of benzyl alcohol as an internal standard for improved accuracy.

    Related Experiment Videos

    Main Results:

    • The described method provides a straightforward and efficient means for total urinary phenol determination.
    • The use of GC-FID ensures sensitive and specific detection of phenol metabolites.
    • Incorporation of an internal standard enhances the precision and reliability of the results.

    Conclusions:

    • This modified procedure offers a practical and robust approach for measuring total urinary phenol.
    • The method is suitable for routine analysis and biomonitoring studies.
    • Accurate phenol measurement is crucial for understanding exposure and potential health effects.