Ribosome purification by isopycnic sedimentation in high density sucrose gradients

The applicability has been investigated of a method for the isolation of cytoplasm (and mitochondrial) ribosomes of various eukaryotes. The procedure is based on centrifugation techniques and includes a sedimentation of the ribosomes in a sucrose gradient of very high density. It was found to be particularly useful with material which contains relatively few ribosomes.