A solid-phase immunofluorescence assay (SIFA) using membrane filters
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Updated: Oct 8, 2026
10:47
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
Acta Histochemica
|January 1, 1982
Abstract:
A solid-phase immunofluorescence assay (SIFA) is presented which allows the demonstration of antibodies against soluble antigens. The test is performed by successively incubating nitrate cellulose discs in microtitration plates with anti-Ig, the sample to be tested and FITC-labeled antigen. The fluorescence intensity of the discs is then measured by using a microfluorometer. The assay is simple, needs a small amount of material and allows to test a large number of samples.
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01:33Enzyme-Linked Immunosorbent Assay
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01:12Immunofluorescence Microscopy
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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