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Glycogen metabolizing enzyme activities in the developing rat liver.

R L Khandelwal

    The International Journal of Biochemistry
    |January 1, 1982
    PubMed
    Summary

    Rat liver glycogen content and key enzyme activities shift dramatically around birth. Glycogen levels decrease postnatally, while synthesis and breakdown enzyme activities fluctuate, indicating complex metabolic regulation during this critical transition.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Metabolic Regulation

    Background:

    • Hepatic glycogen metabolism is crucial for maintaining glucose homeostasis.
    • Significant physiological changes occur in rat liver around the time of birth.
    • Understanding the enzymatic regulation of glycogen synthesis and degradation is vital.

    Purpose of the Study:

    • To investigate the dynamic changes in rat liver glycogen content and the activities of key enzymes involved in its metabolism during the perinatal period.
    • To elucidate the regulatory mechanisms governing glycogen metabolism during the transition from fetal to neonatal life.

    Main Methods:

    • Quantification of liver glycogen content.
    • Assays for glycogen synthase and glycogen phosphorylase activities.
    • Measurement of phosphorylase kinase, protein kinase, and phosphoprotein phosphatase activities in liver extracts.

    Main Results:

    • Liver glycogen content increased during prenatal development and sharply decreased after birth.
    • Glycogen synthase and phosphorylase activities showed prenatal increases, followed by a decline at 12 hours postpartum and subsequent rise.
    • Phosphorylase kinase and protein kinase activities increased postnatally, while phosphoprotein phosphatase activities peaked at birth and then declined.

    Conclusions:

    • Rat liver glycogen metabolism undergoes significant enzymatic adjustments during the perinatal period.
    • The coordinated regulation of glycogen synthesis and degradation enzymes is essential for adapting to extrauterine life.
    • These findings provide insights into the biochemical basis of neonatal glucose homeostasis.

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