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The TRH test in normal subjects: methodological considerations.

P T Loosen, G A Mason, A J Prange

    Psychoneuroendocrinology
    |January 1, 1982
    PubMed
    Summary
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    Thyrotropin-releasing hormone (TRH) stimulates thyroid-stimulating hormone (TSH) and prolactin (PRL) release in both sexes, but PRL response is lower in men. A single blood sample at 30 minutes effectively measures TSH response, while PRL response varies by sex.

    Area of Science:

    • Endocrinology
    • Hormone Regulation
    • Human Physiology

    Background:

    • Thyrotropin-releasing hormone (TRH) is a key regulator of the hypothalamic-pituitary-thyroid axis.
    • Understanding the dynamic response of pituitary hormones to TRH is crucial for diagnosing endocrine disorders.

    Purpose of the Study:

    • To investigate the acute effects of intravenous TRH administration on serum TSH, PRL, GH, T3, T4, and FT4-Index in healthy adults.
    • To compare the TSH and PRL responses to TRH between men and women.
    • To evaluate the correlation between various measures of hormone response (peak, area under the curve, time-point values) and the overall secretory pattern.

    Main Methods:

    • Administration of 500 micrograms of TRH intravenously to 13 normal women and 12 normal men.
    • Serial blood sampling to measure serum concentrations of TSH, PRL, GH, T3, T4, and FT4-Index at baseline and various time points post-injection.

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  • Statistical analysis to determine significant changes, inter-sex differences, and correlations between hormone response parameters.

    • Main Results:

    • TRH injection significantly increased serum TSH and PRL in both sexes (p < 0.01).
    • The prolactin (PRL) response to TRH was significantly lower in men compared to women.
    • Thyroid hormones (T3, T4, FT4-Index) also increased post-TRH, with T3 peaking earlier than T4 and FT4-Index.
    • TSH response correlated strongly with peak TSH and 30-minute TSH values.
    • PRL response correlated with peak PRL and maximum delta PRL, but the 30-minute PRL value was only significantly correlated with the area under the curve in women.

    Conclusions:

    • Baseline and 30-minute serum samples effectively assess the TSH secretory response to TRH in both sexes.
    • The PRL response to TRH is significantly influenced by sex.
    • The 30-minute PRL measurement is a reliable indicator of the overall PRL secretory response in women, but not in men.