[Protein biosynthesis in rat liver subcellular fractions with quantitative and qualitative changes in the composition...

The biosynthesis rate of total proteins of liver subcellular fractions was examined in rats kept for 40 days on a high-carbohydrate diet with the use of starch, and sucrose from 14C-lysine incorporation. It was shown that the majority of subcellular fractions with the exception of the microsomal experienced an increase in the rate of protein biosynthesis. Estimation of the protein content in the fractions allows the conclusion that enhancement of biosynthesis in membrane fractions of external, internal membranes of mitochondria and lysosomes is linked with the increased rate of renewability, while in the fraction of cytoplasm soluble proteins with induction of numerous enzymes because of the increased energy load.