Stimulation of prostaglandin E2 biosynthesis in rat dental pulp explants in vitro by 5-hydroxytryptamine

When excised rat dental pulp tissue was incubated in Krebs solution, prostaglandin E2 (PGE2) was released into the medium. The release was completely blocked by the PG synthetase inhibitor, indomethacin (0.1 mM). Exogenous arachidonic acid (0.01 mM), a substrate of PG synthetase, increased the amount of PGE2 production and the endogenous PGE2 biosynthesis was strongly stimulated by 5-hydroxytryptamine (5-HT) at concentrations higher than 0.01 mM. Bradykinin (0.1 mM) and histamine (0.1 mM) had no effect.