A method to evaluate the biosynthesis of glycosaminoglycans by the aorta of Cynomolgus monkey

The biosynthesis of the glycosaminoglycans (GAGs) was investigated in vitro in the aortic tissue of the Cynomolgus monkey incubated with [14C]glucosamine. With the use of a new micromethod, it was possible to quantify the glycosaminoglycans and their radioactive distribution in the aortic tissue and incubation medium. Labeled and nonlabeled chondroitin 6-sulfate, dermatan sulfate, heparan sulfate and hyaluronic acid were measured. Since the sensitivity of this procedure is between 5 and 20 micrograms of GAGs, as little as 5 mg of dry defatted aortic tissue is sufficient for chemical and radioactive analyses.