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Related Experiment Videos

Transplantation without immunosuppression.

D Faustman, P E Lacy, J M Davie

    Diabetes
    |August 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Successful islet transplantation across major barriers is possible without immunosuppression by eliminating passenger leukocytes. This breakthrough in islet transplantation may also benefit other organ transplants.

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    Area of Science:

    • Immunology
    • Transplantation Biology
    • Endocrinology

    Background:

    • Islet transplantation is a promising treatment for diabetes.
    • Current methods often require lifelong immunosuppression, leading to complications.
    • Eliminating passenger leukocytes offers a potential strategy to reduce transplant rejection.

    Purpose of the Study:

    • To investigate methods for successful islet transplantation without immunosuppression.
    • To validate the passenger leukocyte concept in islet transplantation.
    • To explore the potential application of these findings to human diabetes treatment and other organ transplants.

    Main Methods:

    • In vitro culture of donor islets at low temperature (24°C) with antilymphocyte serum.
    • Culture of megaislets under hyperoxygenation (95% O2).

    Related Experiment Videos

  • Treatment of mouse islets with anti-Ia sera and complement.

    • Main Results:

    • Successful islet allografts and xenografts (rat to mouse) were achieved without immunosuppression.
    • Treatment of islets with anti-Ia sera and complement prevented rejection across major histocompatibility barriers.
    • Ia-positive cells, likely dendritic cells, were identified as primary initiators of transplant rejection.

    Conclusions:

    • Eliminating passenger leukocytes, particularly Ia-positive cells, is crucial for successful islet transplantation.
    • Pretreatment with antisera targeting specific antigens (e.g., HLA-DR) may enable islet transplantation in humans.
    • These findings have broad implications for advancing transplantation of islets and other organs.