Effects of washing on the bacterial flora of the stallion's penis

Six stallions were subjected to extensive cleansing of the penis and prepuce with water, Ivory Soap and water, or Betadine surgical scrub and water. The stallions were all washed for 14 days, and then allowed 14 days respite. This pattern of washing and resting was repeated consecutively. Swabs were taken from all 7 stallions twice weekly and semen was collected once a week for bacteriological examination. All forms of cleansing altered the bacterial flora of the stallion's penis; the Ivory Soap tended to encourage the replacement of the normal flora with coliform organisms, while Betadine favoured the growth of Pseudomonas aeruginosa and Klebsiella spp. This experiment showed that the systematic washing of a stallion's penis will cause the normal flora to be replaced with pathogens and potential pathogens.