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Treatment with antitheta serum affects hemopoietic stem cell HSC proliferation in an in vivo culture system
Marrow cells from normal WBB6F1 +/+ mice were treated in vitro with either anti-Thy 1.2 serum and complement (C') or normal mouse serum (NMS) and C' and then they were cultured in diffusion chambers implanted into peritoneal cavity of Balb/c mice. Following 1-19 days of culture, chamber contents were injected into W/Wv mice to test for either the capacity to form spleen colonies or to cure the W/Wv anemia. In contrast to NMS-treated marrow, anti-Thy 1.2 serum treated marrow hemopoietic stem cells almost completely failed to proliferate in this system. Number of cells per chamber upon termination of culture was less affected.
Marrow cells from normal WBB6F1 +/+ mice were treated in vitro with either anti-Thy 1.2 serum and complement (C') or normal mouse serum (NMS) and C' and then they were cultured in diffusion chambers implanted into peritoneal cavity of Balb/c mice. Following 1-19 days of culture, chamber contents were injected into W/Wv mice to test for either the capacity to form spleen colonies or to cure the W/Wv anemia. In contrast to NMS-treated marrow, anti-Thy 1.2 serum treated marrow hemopoietic stem cells almost completely failed to proliferate in this system. Number of cells per chamber upon termination of culture was less affected.