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Updated: Aug 12, 2026
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
Published on: November 22, 2017
Effect of carrier preimmunization on neutralizing antibody response of rabbits immunized with beta-human chorionic
Abstract:
Antisera to the conjugate of beta-human chorionic gonadotropin and tetanus toxoid (beta hCG-TT) generated in rabbits receiving TT carrier preimmunization were characterized and compared with antisera from rabbits receiving the conjugate without preimmunization. Double immunodiffusion and immunoelectrophoresis were used to identify individual antisera components to the conjugate and its substructures. 125IhCG radioimmunoassay indicated similar antibody titers (P = 0.447) regardless of whether the rabbits were preimmunized with TT. However, bioneutralization of native hCG as measured by inhibition of prepubertal rat uterine weight increase was significantly more effective (P = 0.025) with antisera from rabbits which received carrier presensitization. These results demonstrate that carrier preimmunization provides a method for increasing the efficacy of neutralizing antibody produced against haptenic beta hCG.
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The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...