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Creatinine determined by "high-performance" liquid chromatography.

R T Ambrose, D F Ketchum, J W Smith

    Clinical Chemistry
    |February 1, 1983
    PubMed
    Summary
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    This study introduces a high-performance liquid chromatography method for precise serum creatinine measurement. The new assay shows excellent accuracy and minimal interference, offering a reliable alternative for clinical diagnostics.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biochemistry

    Background:

    • Accurate serum creatinine measurement is crucial for assessing kidney function.
    • Existing methods may face interference or require complex sample preparation.

    Purpose of the Study:

    • To develop and validate a high-performance liquid chromatographic (HPLC) method for serum creatinine determination.
    • To evaluate the accuracy, precision, and interference of the proposed HPLC method.

    Main Methods:

    • Serum samples were analyzed using cation-exchange chromatography.
    • Creatinine was detected by monitoring absorbance at 234 nm.
    • The method was validated against a manual Jaffé-reaction procedure.

    Main Results:

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    • The HPLC method demonstrated close correlation with the manual Jaffé-reaction procedure.
    • No interference was observed from 21 screened endogenous and exogenous substances.
    • High precision was achieved, with overall coefficients of variation (CV) of 1.35% at 138 µmol/L and 0.72% at 539 µmol/L.

    Conclusions:

    • The developed HPLC procedure provides a high-performance, accurate, and precise method for serum creatinine determination.
    • This method offers a robust alternative to existing assays, with minimal interference, enhancing its utility in clinical settings.