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Decrease of E and EA but not EAC rosette formation after incubation of blood lymphocytes in cerebrospinal fluid
Peripheral blood lymphocytes (PBL) were incubated in autologous cerebrospinal fluid (CSF) or Hanks' balanced salt solution (HBSS) for between 5 and 60 min at 37 degrees C. Incubation in CSF caused an increase in cell death as measured by trypan blue inclusion. Incubation in CSF also resulted in a decreased percentage of E and EA rosette-forming cells (RFC)--but not of EAC-RFC--as compared with preincubation levels. Incubation in HBSS did not cause significant changes in rosette formation. Although incubation in CSF resulted in decreased E and EA rosette formation by PBL, lymphocytes isolated from CSF showed increased E and EAC rosette formation as compared with PBL.
Peripheral blood lymphocytes (PBL) were incubated in autologous cerebrospinal fluid (CSF) or Hanks' balanced salt solution (HBSS) for between 5 and 60 min at 37 degrees C. Incubation in CSF caused an increase in cell death as measured by trypan blue inclusion. Incubation in CSF also resulted in a decreased percentage of E and EA rosette-forming cells (RFC)--but not of EAC-RFC--as compared with preincubation levels. Incubation in HBSS did not cause significant changes in rosette formation. Although incubation in CSF resulted in decreased E and EA rosette formation by PBL, lymphocytes isolated from CSF showed increased E and EAC rosette formation as compared with PBL.