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Imaging of melanoma with L-131-labeled monoclonal antibodies
Abstract:
Mouse monoclonal antibodies and Fab fragments specific for p97, a melanoma-associated antigen, were used to image metastatic human melanoma. Preclinical studies in athymic mice showed antigen-specific uptake in melanoma xenografts, and toxicity tests in rabbits gave no evidence for tissue damage after injection of up to 100 times the amount of antibody used in humans. Six patients received 1 mg labeled antibody, and one patient received 1 mg of labeled Fab. No. toxic side effects were observed. All of the six patients had positive scans, visualizing 22 of 25 (88%) of lesions larger than 1.5 cm. In tumors from two patients, greater uptake of p97-specific, versus control IgG and Fab, respectively, was documented by biopsy. Antibodies to mouse immunoglobulin appeared in three patients receiving 1 mg or more of radiolabeled mouse antibody.
Insights
Monoclonal antibodies targeting the melanoma antigen p97 effectively image metastatic melanoma in patients, showing high lesion detection rates and no significant toxicity in early trials.
Area of Science:
- Oncology
- Immunology
- Medical Imaging
Background:
- Metastatic melanoma remains a significant clinical challenge, necessitating improved diagnostic tools.
- p97 is a melanoma-associated antigen identified as a potential target for imaging and therapy.