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Related Experiment Videos

Action potentials in macrophages derived from human monocytes.

F V McCann, J J Cole, P M Guyre

    Science (New York, N.Y.)
    |February 25, 1983
    PubMed
    Summary
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    Mature macrophages exhibit electrical excitability, demonstrating voltage-dependent ion currents. This electrical activity in macrophages could be used to assay their immune functions.

    Area of Science:

    • Immunology
    • Cellular Electrophysiology
    • Membrane Biophysics

    Background:

    • Macrophages are critical immune cells involved in host defense.
    • Understanding macrophage electrophysiology provides insights into cellular function.
    • Previous studies have not fully characterized the intrinsic electrical properties of mature, non-activated macrophages.

    Purpose of the Study:

    • To investigate the intrinsic electrical activity of human macrophages derived from monocytes in vitro.
    • To determine if mature macrophages possess voltage-dependent ion channels.
    • To explore the potential of macrophage electrical signals for functional assays.

    Main Methods:

    • Human blood monocytes were cultured to differentiate into macrophages.
    • Intracellular microelectrode recordings were performed on mature macrophages (6-8 days in culture).

    Related Experiment Videos

  • Computer-assisted data acquisition and analysis were employed to analyze electrical signals.

    • Main Results:

    • Macrophages exhibited resting potentials up to -72 mV and were electrically excitable.
    • Action potentials with characteristic features (slow upstroke, fast downstroke, overshoot) were observed.
    • Repetitive firing was induced by depolarizing currents, indicating voltage-dependent ion conductances.
    • Non-rectifying voltage-current relationships were found for subthreshold currents.

    Conclusions:

    • Mature macrophages possess intrinsic voltage-dependent ion channels, evidenced by their electrical excitability.
    • These findings demonstrate the presence of functional inward and outward currents in macrophage membranes.
    • Macrophage electrical signals offer a novel avenue for developing functional assays for immune responses.