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Related Experiment Videos

Lactoferrin levels in normal human tears.

A Kijlstra, S H Jeurissen, K M Koning

    The British Journal of Ophthalmology
    |March 1, 1983
    PubMed
    Summary
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    Researchers developed an enzyme-linked immunosorbent assay to measure lactoferrin in human tears. This study found normal tear lactoferrin levels are approximately 2.2 mg/ml, unaffected by age or sex.

    Area of Science:

    • Ophthalmology
    • Biochemistry
    • Immunology

    Background:

    • Lactoferrin is a key protein in human tears, playing a role in immune defense.
    • Quantifying lactoferrin levels is important for understanding ocular surface health.

    Purpose of the Study:

    • To develop and validate an enzyme-linked immunosorbent assay (ELISA) for measuring lactoferrin in normal human tears.
    • To determine the normal concentration of lactoferrin in human tears.
    • To investigate factors influencing tear lactoferrin levels, such as collection method, age, and sex.

    Main Methods:

    • Development of an ELISA using commercially available reagents.
    • Tear collection from 38 healthy human subjects using Schirmer tear strips and glass capillaries.
    • Tear gas stimulation was used for capillary collection.

    Related Experiment Videos

  • Measurement of lactoferrin concentration via ELISA.
  • Analysis of tear lactoferrin levels in relation to age, sex, and collection method.

    • Main Results:

    • A reliable ELISA was established for quantifying human tear lactoferrin.
    • The mean lactoferrin concentration in normal human tears was determined to be 2.2 mg/ml.
    • No significant differences in lactoferrin levels were observed based on tear collection method (Schirmer strips vs. glass capillaries).
    • Tear lactoferrin levels showed no correlation with the age or sex of the subjects.
    • Lactoferrin constitutes approximately 25% of the total protein content by weight in human tears.

    Conclusions:

    • The developed ELISA provides a viable method for assessing lactoferrin in human tears.
    • Normal human tear lactoferrin levels are consistently around 2.2 mg/ml.
    • Lactoferrin is a major protein component of human tears, indicating its significant physiological role.