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Activity of lipoprotein lipase in thyroidectomized rats
Biochimica Et Biophysica Acta
|March 1, 1983
Summary
Thyroidectomy in rats increases lipoprotein lipase activity in plasma and adipose tissue but decreases liver lipase activity. This impacts how the body processes fats after heparin injection.
Area of Science:
- Endocrinology
- Lipid Metabolism
- Animal Models
Background:
- Thyroid hormones play a crucial role in regulating lipid metabolism.
- Hypothyroidism can alter the activity of key lipolytic enzymes.
- Understanding these alterations is vital for metabolic research.
Purpose of the Study:
- To investigate the effects of thyroidectomy on lipolytic enzyme activities in rats.
- To examine the impact of thyroidectomy on lipoprotein lipase clearance and heparin elimination.
Main Methods:
- Measurement of postheparin lipolytic activity and lipoprotein lipase activity in plasma.
- Assay of liver lipase activity.
- Quantification of lipoprotein lipase activity in adipose tissue extracts and incubation media.
- In vivo studies using 125I-labeled lipoprotein lipase and heparin clearance.
Main Results:
- Thyroidectomized rats exhibited higher plasma postheparin lipolytic activity and lipoprotein lipase activity compared to controls.
- Liver lipase activity was reduced in thyroidectomized rats.
- Adipose tissue from thyroidectomized rats showed increased lipoprotein lipase activity.
- Thyroidectomy led to more retarded clearance of injected lipoprotein lipase when preceded by heparin and slightly delayed heparin elimination.
Conclusions:
- Thyroidectomy significantly alters the distribution and activity of lipolytic enzymes, particularly lipoprotein lipase.
- These changes in enzyme activity and clearance kinetics in hypothyroid rats have implications for lipid processing and heparin pharmacokinetics.