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Six methods for determining urinary protein compared.

B A Dilena, L A Penberthy, C G Fraser

    Clinical Chemistry
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    Routine urinary protein determination methods are often unsatisfactory. The Ponceau-S technique demonstrated good performance and practicability, making it recommended for laboratory use.

    Area of Science:

    • Clinical Chemistry
    • Analytical Biochemistry

    Background:

    • Routine methods for determining urinary protein levels frequently yield unsatisfactory results in inter-laboratory surveys.
    • Accurate and reliable protein measurement in urine is crucial for diagnosing and monitoring various kidney diseases and other health conditions.

    Purpose of the Study:

    • To compare the performance of six common methods for urinary protein determination.
    • To evaluate methods based on linearity, precision (within-batch and between-batch), comparative bias, and practicability for routine laboratory use.

    Main Methods:

    • Assayed dilutions of human and bovine albumin, serum, and fresh and lyophilized human urine.
    • Evaluated six frequently used urinary protein determination techniques.
    • Assessed key performance characteristics including linearity, precision, bias, and practicability.

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    Main Results:

    • The AACC Selected Method showed poor practicability and precision but good linearity.
    • Sulfosalicylic acid/biuret and Coomassie Brilliant Blue techniques exhibited limitations in linearity and/or precision.
    • Sulfosalicylic acid/sodium sulfate turbidimetric method lacked precision and standardization capability with bovine materials.
    • The Ponceau-S technique demonstrated favorable performance characteristics and practicability.

    Conclusions:

    • Several commonly used urinary protein determination methods are not suitable for routine laboratory use due to issues with precision, linearity, or practicability.
    • The Ponceau-S technique is recommended for routine laboratory determination of urinary protein owing to its good performance and ease of use.