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Glucocorticoids coordinately regulate procollagens type I and type III synthesis.

S Shull, K R Cutroneo

The Journal of Biological Chemistry

|March 10, 1983

Summary

Glucocorticoids, like triamcinolone diacetate, equally inhibit the synthesis of type I and type III procollagens. This coordinate regulation of procollagen synthesis by glucocorticoids is reversible and dose-dependent in skin and lung tissues.

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Area of Science:

Biochemistry
Cell Biology
Pharmacology

Background:

Type I and type III procollagens are crucial extracellular matrix components.
Glucocorticoids are known to affect extracellular matrix metabolism.
The specific effects of glucocorticoids on the synthesis of different procollagen types are not fully understood.

Purpose of the Study:

To investigate the effect of glucocorticoids on the synthesis of type I and type III procollagen.
To determine if glucocorticoids coordinately regulate the synthesis of these two procollagen types.

Main Methods:

Monospecific antibodies against type I and type III procollagens were generated and validated.
Rats were treated with triamcinolone diacetate and pulse-labeled with radioactive proline.

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Procollagens were precipitated using specific antibodies to quantify synthesis rates.

Main Results:

Glucocorticoid treatment resulted in an equal degree of inhibition for both type I and type III procollagen synthesis.
The observed coordinate regulation was reversible, dose-dependent, and time-dependent.
This effect was consistent in both skin and lung tissues.

Conclusions:

Glucocorticoids coordinately regulate the synthesis of type I and type III procollagen in skin and lung.
The synthesis of these two major procollagen types is regulated to the same extent by glucocorticoids.