Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histochemical studies on lectin binding in reactive lymphoid tissues.

S M Hsu, H J Ree

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |April 1, 1983
    PubMed
    Summary

    This study used biotin-labeled lectins to stain reactive lymph nodes and tonsils, identifying distinct binding patterns on lymphoid cells and macrophage-histiocytes. These findings confirm lectin binding sites remain intact after tissue processing, enabling retrospective studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Factors influencing the survival of implant-supported ceramic-ceramic prostheses: A randomized, controlled clinical trial.

    Journal of dentistry·2021
    Same author

    Dissolution activation energy of a fluorapatite glass-ceramic veneer for dental applications.

    Materials science & engineering. C, Materials for biological applications·2020
    Same author

    Variances in the Measurement of Ceramic Powder Properties.

    Journal of research of the National Institute of Standards and Technology·2017
    Same author

    Randomized clinical study of wear of enamel antagonists against polished monolithic zirconia crowns.

    Journal of dentistry·2017
    Same author

    Novel Testing for Corrosion of Glass-Ceramics for Dental Applications.

    Journal of dental research·2017
    Same author

    A novel simple phantom for verifying the dose of radiation therapy.

    BioMed research international·2015

    Area of Science:

    • Immunohistochemistry
    • Glycobiology
    • Cell Biology

    Background:

    • Lectins bind to specific carbohydrate moieties on cell surfaces.
    • Understanding lectin binding patterns in lymphoid tissues aids in cell identification and function studies.
    • Formalin-fixed, paraffin-embedded tissues are commonly used in retrospective studies.

    Purpose of the Study:

    • To investigate the staining patterns of 12 biotin-labeled lectins in reactive lymph nodes and tonsils using the avidin-biotin-peroxidase complex (ABC) method.
    • To determine if lectin binding patterns can differentiate between B and T cells and other cell types.
    • To assess the utility of lectin histochemistry for retrospective studies.

    Main Methods:

    • Application of the avidin-biotin-labeled peroxidase complex (ABC) method.

    Related Experiment Videos

  • Staining of formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils with 12 biotin-labeled lectins.
  • Observation and categorization of lectin binding patterns (membranous, cap-like, cytoplasmic).

    • Main Results:

    • Six lectins (Con A, LCA, PHA, PSA, RCA, WGA) showed binding to lymphoid cells and macrophage-histiocytes, while six did not.
    • Three distinct staining patterns were observed: membranous, cap-like, and cytoplasmic.
    • Cap-like staining was present on lymphocytes in both B and T cell areas, suggesting it is not discriminatory.
    • Membranous staining differentiated B cells (follicles) and germinal center cells, with some lectins also binding T-cells.
    • Cytoplasmic staining in macrophage-histiocytes varied significantly between lectins.

    Conclusions:

    • Carbohydrate moieties on cells retain lectin binding sites after routine tissue processing, validating retrospective studies.
    • Each lectin exhibits a unique reaction pattern, which cannot be predicted solely from cell suspension studies.
    • Lectin histochemistry provides valuable insights into cell surface carbohydrates and can aid in distinguishing cell populations within lymphoid tissues.