Related Experiment Videos
A simple immunoradiometric assay for the terminal SC5b-9 complex of human complement
Journal of Immunological Methods
|February 25, 1983
Summary
Researchers developed a new assay to detect the SC5b-9 complex, a marker of complement activation. This method uses specific antibodies to identify the complex in human serum, aiding in disease diagnosis and research.
Area of Science:
- Immunology
- Biochemistry
- Clinical Chemistry
Background:
- Complement activation via classical or alternative pathways generates the SC5b-9 complex.
- SC5b-9 comprises C5b-C9 complement components and S-protein, expressing unique neoantigens.
- These neoantigens allow immunological distinction from native complement proteins.
Purpose of the Study:
- To develop a method for isolating specific antibodies against C5b-9 neoantigens.
- To create a simple, rapid immunoradiometric assay for detecting the SC5b-9 complex.
- To enable routine screening and experimental detection of SC5b-9.
Main Methods:
- Isolation of radiolabelled antibodies specific to C5b-9 neoantigens.
- Development of an immunoradiometric assay (IRMA) utilizing these antibodies.
- Quantification of SC5b-9 in human serum or plasma samples.
Main Results:
- Successful isolation of specific antibodies to C5b-9 neoantigens.
- A rapid and simple immunoradiometric assay for SC5b-9 was established.
- The assay can detect 3-4 micrograms of SC5b-9 per ml of human serum or plasma.
Conclusions:
- The developed immunoradiometric assay is suitable for routine screening of SC5b-9 in patients.
- The method facilitates the detection of experimentally generated SC5b-9 in vitro.
- This assay provides a valuable tool for studying complement activation and related pathologies.