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The lectin binding affinity of Reed-Sternberg cells
The Journal of Pathology
|February 1, 1983
Summary
This study found fewer lectin-binding sites on Reed-Sternberg cells and other immune cells in Hodgkin
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Hodgkin's disease (HD) is a cancer of the lymphatic system.
- Reed-Sternberg cells are characteristic of HD.
- Lectin binding can indicate cell surface carbohydrate expression.
Purpose of the Study:
- To investigate lectin binding to Reed-Sternberg cells and other immune cells in Hodgkin's disease.
- To compare lectin affinity in HD tissue with normal immune cells.
Main Methods:
- Direct immunofluorescence technique.
- Examination of Reed-Sternberg cells, lymphocytes, and monocytes from ten HD cases.
- Assessment of binding affinity for nine different lectins.
Main Results:
- Reed-Sternberg cells displayed heterogeneous lectin binding.
- A significant decrease in lectin binding was observed in HD cases compared to normal mononuclear cells.
- RCA, PHA, and PNA binding showed particularly marked reductions.
Conclusions:
- A defect in carbohydrate metabolism is suggested in Hodgkin's disease.
- Fewer lectin-binding sites may be present on both Reed-Sternberg cells and mononuclear cells in HD.
- Further quantitative studies are needed to confirm these findings.