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Facilitated calcium diffusion by intestinal calcium-binding protein.

J J Feher

    The American Journal of Physiology
    |March 1, 1983
    PubMed
    Summary
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    The intestinal vitamin D-dependent calcium-binding protein (CaBP) significantly increases calcium transport across membranes. This binding protein enhances calcium diffusion, particularly at physiological concentrations, aiding nutrient absorption.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Physiology

    Background:

    • Calcium is vital for numerous biological processes.
    • Understanding calcium transport mechanisms is crucial for physiology and medicine.
    • The role of specific binding proteins in calcium flux requires elucidation.

    Purpose of the Study:

    • To investigate the effect of intestinal vitamin D-dependent calcium-binding protein (CaBP) on calcium flux.
    • To determine if CaBP enhances calcium transport across a model membrane system.
    • To explore the potential role of CaBP in facilitating calcium diffusion.

    Main Methods:

    • Utilized a flow-through dialysis cell with two dialysis membranes to isolate an aqueous compartment.
    • Measured calcium flux through the isolated compartment.

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  • Assessed the impact of adding intestinal vitamin D-dependent calcium-binding protein (CaBP) on calcium flux.
  • Evaluated the effect of bovine serum albumin as a control.

    • Main Results:

    • Intestinal vitamin D-dependent calcium-binding protein (CaBP) significantly enhanced calcium flux.
    • The enhancement of calcium flux by CaBP was observed at near physiological calcium concentrations (1 x 10(-6) M).
    • Bovine serum albumin did not affect the calcium flux, indicating specificity of CaBP's action.

    Conclusions:

    • Intestinal vitamin D-dependent calcium-binding protein (CaBP) plays a significant role in facilitating calcium transport.
    • CaBP enhances ligand diffusion, acting as a soluble binding protein.
    • These findings suggest CaBP is important for efficient calcium absorption and homeostasis.