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Membrane-bound tubulin in human platelets.

M Steiner

    Biochimica Et Biophysica Acta
    |March 23, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Platelet membranes contain tubulin, a protein previously thought to be only in the cytoplasm. This discovery was confirmed using various biochemical and immunological methods, identifying tubulin within the platelet membrane structure.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hematology

    Background:

    • Platelets are anucleated cell fragments crucial for hemostasis.
    • The presence and function of specific proteins within platelet membranes are key to understanding platelet activity.
    • Tubulin, a major component of microtubules, has primarily been studied in the cytoplasm of nucleated cells.

    Purpose of the Study:

    • To investigate the presence and characteristics of colchicine-binding activity within platelet membranes.
    • To determine if the observed activity is due to tubulin and to characterize its properties.
    • To confirm the existence of tubulin in the platelet membrane.

    Main Methods:

    • Solubilization of platelet membranes using detergents (Triton X-100, Lubrol PX, Nonidet P-40).

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  • Measurement of colchicine and vinblastine binding to platelet membranes under equilibrium conditions.
  • Identification of membrane tubulin via acrylamide gel electrophoresis and immunologic precipitation.

    • Main Results:

    • Colchicine-binding activity was detected in platelet membranes, distinct from cytoplasmic contamination.
    • Detergents like Triton X-100 and Nonidet P-40 effectively solubilized this activity.
    • Platelet membrane proteins exhibited higher binding affinity for vinblastine than colchicine.
    • Acrylamide gel electrophoresis and immunologic precipitation confirmed the presence of tubulin with heat-resistant properties similar to cytoplasmic tubulin.

    Conclusions:

    • Tubulin is definitively present in the platelet membrane.
    • Platelet membrane tubulin exhibits distinct characteristics, including enhanced heat resistance.
    • This finding expands the known cellular localization and potential roles of tubulin.