Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cubic protein-monoolein-water phase.

B Ericsson, K Larsson, K Fontell

    Biochimica Et Biophysica Acta
    |March 23, 1983
    PubMed
    Summary

    Researchers identified a cubic lipid-protein-water phase, finding that proteins with high electrostatic repulsion, indicated by an isoelectric point far from pH 7, favor its formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of substitutional N on important chemical vapor deposition diamond growth steps.

    The journal of physical chemistry. A·2009
    Same author

    Repeated exposure to organic material alters inflammatory and physiological airway responses.

    The European respiratory journal·2009
    Same author

    Expression of the class I interferon-related MxA protein in temporal arteries in polymyalgia rheumatica and temporal arteritis.

    Scandinavian journal of rheumatology·2009
    Same author

    Effect of formoterol with or without budesonide in repeated low-dose allergen challenge.

    The European respiratory journal·2009
    Same author

    Characterization of PrEST-based antibodies towards human Cytokeratin-17.

    Journal of immunological methods·2008
    Same author

    Temporal trends of HLA genotype frequencies of type 1 diabetes patients in Sweden from 1986 to 2005 suggest altered risk.

    Acta diabetologica·2008

    Area of Science:

    • Biophysics
    • Materials Science
    • Protein Chemistry

    Background:

    • Lipid-protein interactions are crucial for biological systems.
    • Cubic lipid phases are complex self-assembled structures with potential applications.
    • Understanding protein behavior within these phases is key to their function and stability.

    Purpose of the Study:

    • To identify and characterize a novel cubic monoacylglycerol-protein-water phase.
    • To investigate the thermal stability of proteins within this lipid-protein cubic phase.
    • To determine the factors influencing protein incorporation into this specific cubic phase.

    Main Methods:

    • Low-angle X-ray diffraction was used to identify the cubic phase.
    • Differential scanning calorimetry assessed the thermal stability of lysozyme within the phase.
    • Ternary phase diagrams (monoolein/lysozyme/water) were analyzed.

    Main Results:

    • A cubic monoacylglycerol-protein-water phase was successfully identified.
    • Protein molecules are proposed to reside within the water channels of the cubic structure.
    • Protein ability to form this phase is enhanced by a high isoelectric point (pI) in salt-free conditions, suggesting electrostatic repulsion is a key factor.

    Conclusions:

    • The study demonstrates the formation of a stable cubic lipid-protein-water phase.
    • Protein location within the water channels of the cubic structure influences phase formation.
    • Electrostatic properties of proteins, specifically their isoelectric point, significantly impact their ability to form such lipid-protein phases.

    Related Experiment Videos