Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Germ cell nucleosomes contain remodeled core protein complex.

Y M Bhatnagar, R D Faulkner

    Biochimica Et Biophysica Acta
    |January 20, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Using the scanning electron microprobe and secondary ion mass spectrometry to locate 14C- and 13C-labelled plant residues within soil aggregates.

    Scanning·2006
    Same author

    Using the scanning electron microprobe and secondary ion mass spectrometry to locate 14C and 13C labelled plant residues within soil aggregates.

    Scanning·2006
    Same author

    Donor lymphocyte infusions can result in sustained remissions in patients with residual or relapsed lymphoid malignancy following allogeneic haemopoietic stem cell transplantation.

    Bone marrow transplantation·2005
    Same author

    Analysis of detached human kinetochores.

    Chromosoma·2003
    Same author

    Determination of travoprost and travoprost free acid in human plasma by electrospray HPLC/MS/MS.

    Journal of pharmaceutical and biomedical analysis·2002
    Same author

    Increased accessibility of the N-terminus of testis-specific histone TH2B to antibodies in elongating spermatids.

    Molecular reproduction and development·1995
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Mammalian spermatogenesis involves significant remodeling of nucleosomal core proteins. Testis-specific histones and varying DNA lengths were identified in seminiferous tubule subpopulations, indicating dynamic changes during sperm development.

    Area of Science:

    • Molecular Biology
    • Reproductive Biology
    • Genetics

    Background:

    • Nucleosomes are fundamental units of DNA packaging in eukaryotes.
    • Spermatogenesis requires extensive chromatin modifications for genome regulation.
    • Understanding histone composition and DNA organization is crucial for reproductive health.

    Purpose of the Study:

    • To investigate the composition of nucleosomal histones in different seminiferous tubule subpopulations.
    • To analyze the DNA fragment sizes associated with nucleosomes during spermatogenesis.
    • To elucidate the extent of nucleosomal remodeling during mammalian sperm development.

    Main Methods:

    • Electrophoretic analysis of nucleosomal histones under varying urea concentrations (6.25 M and 2.5 M).
    • Size analysis of DNA segments within nucleosomes from MN1 and MN2 subpopulations.

    Related Experiment Videos

  • Comparison of nucleosome composition between liver and tubule cells.

    • Main Results:

    • Identified testis-specific histone H2S, H1, and protein 'A' alongside somatic histones in the core complex.
    • Observed DNA segments of 150-160 bp in MN1 subpopulation nucleosomes.
    • Found approximately 180 bp DNA fragments in MN2 subpopulation nucleosomes from both liver and tubule cells.

    Conclusions:

    • Mammalian spermatogenesis is characterized by extensive remodeling of the nucleosomal core protein complex.
    • Distinct histone compositions and DNA lengths characterize different nucleosome populations during sperm development.
    • These findings provide insights into the dynamic nature of chromatin structure during male gametogenesis.